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. 2021 Dec 17;16(12):e0261230. doi: 10.1371/journal.pone.0261230

Fig 2. Validation of a pooled sample testing strategy for campus-wide SARS-CoV-2 surveillance.

Fig 2

(A) 10:1 linear pooling strategy. Upto 10 individual sample racks (children) are combined into a single pooled sample (parent) rack. (B) UCSC multiplex N1 assay on 30 individual or pooled positive samples. (C) UCSC multiplex N2 assay on 30 individual or pooled positive samples. (D) Analysis of pooled positive samples with the UCSC multiplex or comparator assay. N1 amplicon (blue) and N2 amplicon (red). (E) Overall performance of the UCSC multiplex assay on thousands of clinical, surveillance and pooled surveillance samples. (F) Turnaround time for both clinical and surveillance samples, rounded to the nearest hour.