Table 1. The limit of detection for the UC Santa Cruz Multiplex SARS-CoV-2 assay.

Dilution (vg/uL)	N1 positive	N2 positive	Cq Mean N1	Cq Mean N2	Cq Mean RNase P
10	3/3	3/3	31.66	30.89	25.02
5	3/3	3/3	32.69	31.84	25.10
2.5	3/3	3/3	33.98	33.33	25.11
1.25	3/3	3/3	34.25	33.50	24.91
0.625	3/3	3/3	35.18	34.63	24.99
0.3125	2/3	2/3	36.46	35.41	24.99
0.156	1/3	1/3	37.33	36.89	24.65
0.078	0/3	0/3	ND	ND	24.39
0.039	1/3	1/3	37.18	35.90	24.93
0	0/3	0/3	ND	ND	24.65