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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Blood Cells Mol Dis. 2021 Nov 3;92:102624. doi: 10.1016/j.bcmd.2021.102624

Figure 2. Deletion of the Mir-99b/let7e/125a cluster lower blood platelet counts.

Figure 2.

A. Schema showing experimental design. Mice were generated with the targeted allele containing loxP sites flanking the Mir-99b/let7e/125a cluster. These mice were bred with PFA-Cre mice resulting in mice with MKs lacking the Mir-99b/let7e/125a cluster. B. Fold change of mmu-miR-99b-5p, mmu-let-7e-5p and mmu-miR-125a-5p in leukocyte depleted platelets (LDP) isolated from Mir-99b/let-7e/125a −/− mice (n=6) compared to their littermates (n=6), as assessed by TaqMan advance miRNA assay. miR-320a-5p (expression did not change in knockout vs littermates, data not shown) was used as a housekeeping gene for fold change calculation. C. Peripheral blood platelet count measured by Hemavet for Mir-99b/let7e/125a−/− mice (n=23) or littermates (n=20) (8 weeks old, and gender matched).