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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Blood Cells Mol Dis. 2021 Nov 3;92:102624. doi: 10.1016/j.bcmd.2021.102624

Figure 4. MK/platelet specific loss of Mir-99b/let7e/125a does not alter platelet function or in vivo thrombosis.

Figure 4.

A-B. Washed platelets from Mir-99b/let7e/125a −/− (n=8) or littermates (n=11) were activated with 50, 100 or 150 μM AYPGKF and 1, 30 or 100 ng/ml collagen receptor peptide (CRP). A. JON/A binding and B. P-selectin was measured by flow cytometry. C. Washed platelets from Mir-99b/let7e/125a −/− (n=8) or littermates (n=11) were activated with AYPGKF (30 and 100 μM) or collagen (30 and 100 ng). Aggregation was measured using Chronolog aggregometer and plotted (n=3 to 7). D-E. Phalloidin staining of washed platelets isolated from Mir-99b/let7e/125a −/− or littermates, that were adhered on D. immobilized fibrinogen or E. collagen (n=3 each). F. Pulmonary thromboembolism was induced by injecting Mir-99b/let7e/125a −/− mice or littermates with collagen and epinephrine (n=10 each). Time to death was monitored and plotted.