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. 2021 Nov;113(6):3842–3850. doi: 10.1016/j.ygeno.2021.09.012

Fig. 3.

Fig. 3

Assessment of the role of Nae1 and Cdh1 in the replication of IPNV in Atlantic salmon cells. A) IPNV viral load at 96 and 120 hpi in control and nae1 KO SHK-1 infected with IPNV at an MOI of 0.01. Relative expression levels of IPNV VP2 to ef1a in cells were normalised to time-matched control SHK-1 cells. B) Infectivity of IPNV in supernatant at 120 hpi in control and nae1 KO SHK-1 infected with IPNV at an MOI of 0.01 was assessed by TCID50/mL on naïve CHSE-214 cells. C) Infectivity of IPNV in cells and supernatant at 120 hpi in SHK-1 cells treated with 100 nM, 1 μM and 5 μM of MLN4924 or DMSO only and infected with IPNV at an MOI of 0.01 was assessed by TCID50/mL on naïve CHSE-214 cells. D) IPNV viral protein in supernatant of SHK-1 cells treated with 100 nM MLN4924 and infected at an MOI of 0.01 at 120 hpi was analysed by western blotting using an antibody against IPNV viral proteins. E) IPNV viral load at 120 hpi in control and cdh1 KO SHK-1 infected with IPNV at an MOI of 0.01. Relative expression levels of IPNV VP2 to ef1a in cells were normalised to time-matched control SHK-1 cells. F) Infectivity of IPNV in supernatant at 120 hpi in control and cdh1 KO SHK-1 infected with IPNV at an MOI of 0.01 was assessed by TCID50/mL on naïve CHSE-214 cells. Significance levels denoted: * P < 0.05, ** P < 0.01, **** P < 0.0001.