Composition	Stock solution/storage
10 mM HEPES-NaOH (pH 7.5)	0.5 M/RT
10 mM NaCl	5 M/RT
5 mM MgCl2	1 M/RT
0.1% Igepal CA-630	20%/RT
0.5 mg/ml heparin	30 mg/ml heparin (Sigma H4784) in nuclease-free H2O.Filter (0.22 µm)/-20°C
600 U /ml RNasin ribonuclease inhibitor (add just before using the buffer)	RNasin (Promega N2115) 40 U/µl/-20°C