Composition	Stock solution/storage
20 mM HEPES-NaOH (pH 7.5)	0.5 M/RT
200 mM NaCl	5 M/RT
4 mM EDTA	0.5 M/RT
0.1% Igepal CA-630	20%/RT
0.04% sodium deoxycholate	10%/RT
4 mM imidazole	1 M/RT
0.1 mg/ml heparin	30 mg/ml heparin (Sigma H4784) in nuclease-free H2O. Filter (0.22 µm)/-20°C
1 mM DTT	1 M/-20°C
cOmplete protease inhibitor (1/100)	1 tablet of cOmplete dissolved in 10 ml of nuclease-free H2O/-20°C
600 U /ml RNasin ribonuclease inhibitor (add just before using the buffer)	RNasin 40 U/µl −20°C