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(a) Right, an unused unstained aligner. Left, used aligner after staining with gentian violet. R1–R6 are the sites that were scanned and measured in the TINA software. (b) Top, unused, unstained aligner's segment used to calibrate background noises. Bottom, a used aligner's segment. In each tooth, the three sites were delineated in the TINA software for photodensitometric measurements.
