| Recombinant DNA reagent |
PARP1 (pACEBac1) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
PARP2 (pACEBac1) (plasmid) |
Addgene |
111,574 |
|
| Recombinant DNA reagent |
HPF1 (pET30) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
HPF1D283A (pET30) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
ARH3 (pET30) (plasmid) |
Addgene |
111,578 |
|
| Recombinant DNA reagent |
PARG (pET30) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
ALC1 macrodomain (pET30) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
ALC1 ATPase domain (pET30) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
ALC1 (pACEBac1) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
CHD4 (pACEBac1) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
Histone H2A (pET30) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
Histone H2B (pET30) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
Histone H2B truncated (pET30) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
Histone H3 (pET30) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
Histone H3 truncated (pET30) (plasmid) |
This study |
|
See Materials and methods |
| Recombinant DNA reagent |
Histone H4 (pET30) (plasmid) |
This study |
|
See Materials and methods |
| Sequence-based reagent |
DNA oligonucleotides |
This study |
|
See Supplementary file 10
|
| Antibody |
Antibodies used for western blot |
|
|
See Supplementary file 11
|
| Strain, strain background (Escherichia coli) |
Mach1 (Escherichia coli) |
ThermoFisher |
C862003 |
|
| Strain, strain background (Escherichia coli) |
DH10Bac |
ThermoFisher |
10361012 |
|
| Strain, strain background (Escherichia coli) |
Rosetta 2 |
Sigma Aldrich |
714,023 |
|
| Cell line (Spodoptera frugiperda) |
Sf9 |
ThermoFisher |
11496015 |
|
| Cell line (Homo sapiens) |
HEK293T |
ATCC |
CRL-3216 |
|
| Peptide, recombinant protein |
FLAG peptide |
GenScript |
RP10586-1 |
Sequence:DYKDDDDK |
| Commercial assay or kit |
Gibson Assembly Master Mix |
New England Biolabs (NEB) |
E2611S |
|
| Commercial assay or kit |
iTaq Universal SYBR Green Supermix |
BioRad |
1725121 |
|
| Commercial assay or kit |
Pierce BCA Protein Assay Kit |
ThermoFisher |
23,227 |
|
| Chemical compound, drug |
β-Nicotinamide adenine dinucleotide hydrate |
Sigma Aldrich |
N0632
|
|
| Chemical compound, drug |
Adenosine triphosphate |
Sigma Aldrich |
A26209-1G |
|
| Chemical compound, drug |
HisPur Ni-NTA Resin |
ThermoFisher |
88,223 |
|
| Chemical compound, drug |
Anti-FLAG M2 Magnetic beads |
Millipore Sigma |
M8823-5ML |
|
| Chemical compound, drug |
Phusion High-Fidelity DNA Polymerase |
NEB |
M0530L |
|
| Chemical compound, drug |
PstI enzyme |
NEB |
R0140M |
|
| Chemical compound, drug |
DraIII enzyme |
NEB |
R3510S |
|
| Chemical compound, drug |
T4 Polynucleotide Kinase |
NEB |
M0201S |
|
| Chemical compound, drug |
T4 DNA Ligase |
NEB |
M0202L |
|
| Chemical compound, drug |
Cellfectin II Reagent |
ThermoFisher |
10362100 |
|
| Chemical compound, drug |
Sf-900 II SFM Media |
ThermoFisher |
10902096 |
|
| Chemical compound, drug |
Fetal Bovine Serum |
ThermoFisher |
10438026 |
|
| Chemical compound, drug |
DMEM, high glucose, pyruvate |
ThermoFisher |
11995065 |
|
| Chemical compound, drug |
Penicillin-Streptomycin |
ThermoFisher |
15140122 |
|
| Chemical compound, drug |
Lipofectamine 2000 |
ThermoFisher |
11-668-019 |
|
| Chemical compound, drug |
Puromycin dihydrochloride |
ThermoFisher |
A1113803 |
|
| Chemical compound, drug |
Hydroxylamine hydrochloride |
Sigma Aldrich |
379,921 |
|
| Chemical compound, drug |
N,N-dimethylformamide |
Oakwood Chemical |
046776 |
|
| Chemical compound, drug |
Dichloromethane |
Oakwood Chemical |
035912 |
|
| Chemical compound, drug |
Trifluoroacetic acid |
Oakwood Chemical |
102,164 |
|
| Chemical compound, drug |
Acetonitrile |
Oakwood Chemical |
099891 |
|
| Chemical compound, drug |
Formic Acid, LC/MS Grade |
Thermo Fisher |
A117-50 |
|
| Chemical compound, drug |
N,N'-Diisopropylcarbodiimide |
Oakwood Chemical |
M02889 |
|
| Chemical compound, drug |
Ethyl cyanohydroxyiminoacetate |
Oakwood Chemical |
043278 |
|
| Chemical compound, drug |
N,N-Diisopropylethylamine |
Sigma Aldrich |
496,219 |
|
| Chemical compound, drug |
Thionyl chloride |
Sigma Aldrich |
230,464 |
|
| Chemical compound, drug |
Triisopropylsilane |
Sigma Aldrich |
233,781 |
|
| Chemical compound, drug |
Piperidine |
Sigma Aldrich |
104,094 |
|
| Chemical compound, drug |
PyAOP |
Oakwood Chemical |
024898 |
|
| Chemical compound, drug |
Econo-Pac Chromatography Columns |
Bio-Rad |
7321011 |
|
| Chemical compound, drug |
Sodium nitrite |
Sigma Aldrich |
237,213 |
|
| Chemical compound, drug |
TCEP |
GoldBio |
TCEP50 |
|
| Chemical compound, drug |
Sodium 2-mercaptoethanesulfonate |
Sigma Aldrich |
63,705 |
|
| Chemical compound, drug |
2,2,2-Trifluoroethanethiol |
Sigma Aldrich |
374008–1 G |
|
| Chemical compound, drug |
Trityl-OH ChemMatrix |
Biotage |
7-420-1310 |
|
| Chemical compound, drug |
Rink-Amide-ChemMatrix |
Biotage |
7-600-1310 |
|
| Chemical compound, drug |
Fmoc-Ala-OH |
Oakwood Chemical |
M03347 |
|
| Chemical compound, drug |
Fmoc-Arg(pbf)-OH |
Oakwood Chemical |
M03398 |
|
| Chemical compound, drug |
Fmoc-Thr(tBu)-OH |
Oakwood Chemical |
M03389 |
|
| Chemical compound, drug |
Fmoc-Lys(Boc)-OH |
Oakwood Chemical |
M03419 |
|
| Chemical compound, drug |
Fmoc-Gln(trt)-OH |
Combi-Blocks |
QB-0626 |
|
| Chemical compound, drug |
Fmoc-Ser(tBu)-OH |
Combi-Blocks |
SS-0149 |
|
| Chemical compound, drug |
Fmoc-Gly-OH |
Oakwood Chemical |
M03361 |
|
| Chemical compound, drug |
Fmoc-Leu-OH |
Oakwood Chemical |
M03365 |
|
| Chemical compound, drug |
Fmoc-Pro-OH |
Oakwood Chemical |
M03372 |
|
| Chemical compound, drug |
Fmoc-Cys(Trt)-OH |
Oakwood Chemical |
M03395 |
|
| Chemical compound, drug |
Boc-L-thiazolidine-4-carboxylic acid |
Combi-Blocks |
SS-9673 |
|
| Chemical compound, drug |
5 (6)-Carboxyfluorescein |
Sigma Aldrich |
21877–1 G-F |
|
| Chemical compound, drug |
SEA-PS Resin |
Iris Biotech |
8551520001 |
|
| Software, algorithm |
Prism |
GraphPad |
|
|
| Software, algorithm |
Fiji Image J |
Open source |
|
|
| Software, algorithm |
Microsoft Office |
Microsoft |
|
|
| Software, algorithm |
Adobe Creative Cloud |
Adobe |
|
|
| Software, algorithm |
SnapGene |
GSL Biotech LLC |
|
|
| Software, algorithm |
ChemDraw |
PerkinElmer |
|
|
| Software, algorithm |
BLAST |
NCBI |
|
|
| Other |
Liberty Blue Automated Peptide Synthesizer |
CEM |
925,600 |
|
| Other |
Agilent 1,260 Infinity II with quaternary pump and variable wavelength detector |
Agilent |
G7111B/G7114A |
|
| Other |
Agilent 1,260 Infinity II with preparatory pump and variable wavelength detector |
Agilent |
G7161A/G7114A |
|
| Other |
Agilent LC/MSD |
Agilent |
G6125BA |
|
| Other |
X500B QTOF |
Sciex |
X500B QTOF |
|
| Other |
300 SB-C18, 4.6 × 100 mm, 3.5 um |
Agilent |
861973–902 |
|
| Other |
XBridge Peptide C18 column, 5 um, 4.6 mm x 150 mm |
Waters |
186003624 |
|
| Other |
XBridge Peptide C18 column, 5 um, 10 mm x 250 mm |
Waters |
186008193 |
|
| Other |
XBridge Peptide C18 column, 10 um, 19 mm x 250 mm |
Waters |
186003673 |
|
| Other |
AKTA pure 25 L |
GE Healthcare |
29018224 |
|
| Other |
AKTA start |
GE Healthcare |
29237234 |
|
| Other |
HiLoad 16/60 Superdex 200 pg |
GE Healthcare |
28989335 |
|
| Other |
Superdex 200 Increase 10/300 GL |
GE Healthcare |
28990944 |
|
| Other |
HiTrap Heparin HP |
GE Healthcare |
17040701 |
|
| Other |
5% Criterion TBE Polyacrylamide Gel |
Bio-Rad |
3450048 |
|
| Other |
4%–12% Criterion XT Bis-Tris Protein Gel, |
Bio-Rad |
3450124 |
|
| Other |
Cytation 5 Imaging reader |
Biotek |
17103120 |
|
| Other |
Green FP filter set: EX 485/20 | EM 528/20 | DM 510, polarizers |
Biotek |
8040561 |
|
| Other |
Chemidoc MP Imaging System |
Bio-Rad |
734BR2154 |
|
| Other |
CFX384 Real Time System C1000 Touch Thermal cycler |
Bio-Rad |
786BR02877 |
|
