Figure 5. Validation of preexisting GRNs and putative novel function of specific gene regulatory modules.

(A) Dotplot showing the mRNA localization of genes involved in the homing of small micromeres to the coelomic pouch and novel apical plate domain. (B) Dotplot of aboral ectoderm regulatory module genes showing novel apical plate expression. (C) Pre-gastrula gene regulatory network enriched in skeletal cells of the sea urchin pluteus larva. Asterisks indicate larval genes involved in biomineralization, putative members of this GRN.

Figure 5—figure supplement 1. Reconstruction of the molecular signature of the gut at a single-cell resolution.

Dotplot showing the regionalized average expression of marker genes labeling specific endodermal domains. The scRNA-seq prediction recapitulates the previously described compartmentalization of gene expression along the digestive tract of the 3 dpf sea urchin larva.