Figure 5.

In vitro expansion of CD4⁺CD25⁺Foxp3⁺ and CD4⁺IL10⁺ cell populations from lymph nodes of A/J, BALB/c, or C57BL/6 naive mice. Cells from cervical, axillary, and inguinal lymph nodes were cultured for 72 h in the presence of IL-2 or IL-2 + TGF-β + anti-CD3 + anti-CD28, and subpopulations of CD4⁺CD25⁺Foxp3⁺ (a) or CD4⁺IL-10⁺ (b) were analyzed by flow cytometry. Values represent mean ± SEM of 4-5 animals per group. ⁺⁺⁺P < 0.001 as compared to the same mouse strain cells that received only IL-2. ^∗∗P < 0.01 and ^∗∗∗P < 0.001 are comparisons of different mouse strains stimulated with IL-2 + TGF-β + anti-CD3 + anti-CD28.