Figure 6.

In vitro expansion of CD4⁺IL4⁺, CD4⁺IL17⁺, CD4⁺CD25⁺Foxp3⁺, and CD4⁺IL10⁺ cell populations from lymph nodes of A/J, BALB/c, or C57BL/6 OVA-challenged mice. Cells from mediastinal lymph nodes were cultured for 72 h in the presence IL-2, IL-2 + OVA, or IL-2 + OVA + TGF-β, and subpopulations of CD4⁺IL4⁺ (a) and CD4⁺IL17⁺ (b) CD4⁺CD25⁺Foxp3⁺ (c) or CD4⁺IL10⁺ (d) were analyzed by flow cytometry. IL-10 production in the supernatant of cultured cells was detected by ELISA (e). Values represent mean ± SEM of 4-5 animals per group. ^#P < 0.05 and ^###P < 0.001 as compared to the same mouse strain group that received only IL-2. ⁺⁺P < 0.01 and ⁺⁺⁺P < 0.001 as compared to the same mouse strain group that received IL-2 + OVA. ^§P < 0.05 and ^§§§P < 0.001 as compared to the same mouse strain group that received IL-2 + OVA + TGF-β. ^&P < 0.05 as compared to the same strain between different stimulus. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001. Correlation between frequency of CD4⁺IL10⁺ cells and IL-10 production in cultures stimulated with OVA and TGF-β (f). Pseudo-r = 0.97, P = 0.02 by univariate analysis.