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. 2021 Dec 10;2021:8817421. doi: 10.1155/2021/8817421

Figure 6.

Figure 6

Butyrate does not significantly impact bone healing outcomes while rifampicin and levofloxacin may delay bone healing in a murine osteotomy model. Bone healing outcomes of operated mice were assessed 10 days, 18 days, and 21 days (euthanasia) following osteotomy. (a) Failure load of contralateral, unoperated femora was determined by a four-point-bending test. Data shown are means (n = 3‐4) ± SEM. (b) Procollagen type I C-terminal propeptide (PINP) in serum was determined 10 days and 21 days after osteotomy. Data shown are means (n = 7‐10) ± SEM. (c) Bone volume was measured by μCT 21 days after osteotomy. Data shown are means (n = 6‐10) ± SEM. (d) Quantification of calcium deposition at osteotomy site 10 days after osteotomy (calcein green injection) and 18 days after osteotomy (xylenol orange injection). Data shown are mean fluorescent intensities (n = 4‐5) ± SEM. (e) Representative fluorescent images showing calcein green and xylenol orange labelling of exposed calcium at osteotomy site. Scale bar = 500 μm, ∗p < 0.05.