TABLE 1.
Effect of pretreatment of C. immitis cultures on accuracy of AccuProbe test
| C. immitis strain no. | Avg RLUa
|
||||||
|---|---|---|---|---|---|---|---|
| AccuProbe protocolb | Boiled culturesc | Mixed culturesd
|
37.5% Formaldehydee | 10% Formaldehyde
|
|||
| A. fumigatus | C. albicans | Unwashede | 3 Washesf | ||||
| NYS 104-94 | 699,787 (+) | 745,639 (+) | 62,191 (+) | 741,536 (+) | 2,869 (−) | 6,887 (−) | 18,499 (−) |
| NYS 498-97 | 717,437 (+) | 525,529 (+) | 619,501 (+) | 277,748 (+) | 6,685 (−) | 3,942 (−) | 52,317 (±) |
| NYS 56-98 | 484,562 (+) | 398,424 (+) | 314,104 (+) | 624,013 (+) | 3,014 (−) | 1,662 (−) | 47,769 (±) |
| NYS 89-99 | 702,192 (+) | 203,661 (+) | 811,376 (+) | 709,925 (+) | 2,178 (−) | 4,895 (−) | 15,200 (−) |
| NYS 371-99 | 750,192 (+) | 203,661 (+) | 455,968 (+) | 803,618 (+) | 2,178 (−) | 4,895 (−) | 186,675 (+) |
From two experiments. Interpretations of results are in parentheses; ±, borderline positive.
Includes lysis at 95°C for 5 min. The positive control was C. immitis NYS 1442-98 (RLU = 768,904 ± 110,916); the negative control was Arthrographis sp. strain NYS 721-95 (RLU = 3,882 ± 1,075). Values are from 10 independent determinations.
Twenty minutes in boiling water bath, storage at 6°C for 48 h, and processing as per AccuProbe protocol.
Mixed with 106 conidia or yeast cells and processed as per AccuProbe protocol.
Treated with formaldehyde for 60 min and processed as per AccuProbe protocol.
Formaldehyde treatment followed by three rinses with deionized water and processing as per AccuProbe protocol.