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. 2021 Nov 22;23(12):1287–1298. doi: 10.1038/s41556-021-00792-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Representative images of the RNAPI foci (RPA194) and levels of global transcription (EU immunofluorescence) in WT and XRCC1^−/− U2OS cells stably transfected with either empty vector (EV) or expression construct encoding full length C-terminal histidine-tagged XRCC1 (XRCC1^WT), following mock treatment or at the indicated times after treatment with 1 mM H₂O₂ for 20 min. Scale bars, 10 μm. b and c, Quantification of the RNAPI foci (RPA194) and levels of global transcription (EU immunofluorescence) shown in (a). Data are means (±s.e.m.) of three independent experiments, and statistically significant differences were determined by two-way ANOVA with Tukey’s multiple comparisons test (p values are indicated). d, Immunoblot of RNAPII hyperphosphorylation in WT U2OS cells, XRCC1^−/− U2OS cells, and XRCC1^−/− U2OS cells stably transfected with either empty vector (EV) or expression construct encoding full-length histidine-tagged XRCC1 (XRCC1^WT), following mock treatment or 2 h after treatment with 1 mM H₂O₂ for 20 min. A representative blot from one of three independent experiments is shown.

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