Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 22;23(12):1287–1298. doi: 10.1038/s41556-021-00792-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 6 — a, Representative images of the RNAPI foci (RPA194) and levels of global transcription (EU Immunofluorescence) in WT and XRCC1^−/− RPE-1 cells following mock treatment or at the indicated times after treatment with 250 μM H₂O₂ for 5 min. b, Quantification of the RNAPI foci (RPA194) shown in (a). Data are means (±s.e.m.) of three independent experiments, and statistically significant differences were determined by two-way ANOVA with Sidak’s multiple comparisons test (p values are indicated). c and d, Immunoblot of PARP1 and/or ADP-ribose levels in total cell extract (panel c) and following ADP-ribose immunoprecipitation (panel d) prepared from WT and XRCC1^−/− RPE-1 cells following mock treatment or at 2 h after treatment with 250 μM H₂O₂ for 5 min. Representative blots from one of three independent experiments are shown. e, Immunoblot of NTH1 in WT and XRCC1^−/− RPE-1 cells treated for 72 h with 10 nM siRNA against NTH1. A representative blot from one of two independent experiments is shown.

Source data