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. 2021 Nov;2(11):e617–e626. doi: 10.1016/S2666-5247(21)00150-6

Figure 5.

Figure 5

Pilot development of a point of care immunochromatographic test for diagnosis of urogenital schistosomiasis using serum

(A) Representative tests showing positive results of differing band intensities. (B) Schematic showing positive (red boxes) and negative (white boxes) results for MS3_01370 or Sh-TSP-2 immunochromatographic test using ELISA-validated serum samples from the Gabon cohort (high, n=10; low, n=22; egg− and CAA+, n=4; egg− and CAA−, n=14; non-endemic, n=10). Strips were coated with either MS3_01370 or Sh-TSP-2 at the test line to facilitate capture and detection of anti-MS3_01370 or anti-Sh-TSP-2 IgG in serum added to the sample reservoir. Appearance of a band at the test and control lines was considered a positive result and a band at the control line only was considered a negative result. Test bands were given a score from least (+1) to most (+4) intense and a score of 0 was given to a negative result. Two independent readers had to agree on a test result. Every test performed was valid, as confirmed by the appearance of a band at the control line. Samples have been sorted from left to right by decreasing egg burden.