Figure 1.

Characterization of cells transfected to express human ABCB1, zebrafish Abcb4 or Abcb5. Whole cell lysates were prepared from HEK293 cells transfected with empty vector (Vector), human ABCB1 (MDR-19), zebrafish abcb4 (Dr Abcb4) or abcb5 (Dr Abcb5), subjected to polyacrylamide gel electrophoresis and transferred to nitrocellulose. Blots were probed with anti-FLAG antibody and beta actin (a) or anti-ABCB1 antibody C219 and beta-actin (b). Sections in (a) were cropped from the blot in Supplementary Fig. S1a; sections in (b) were cropped from the blot in Supplemental Fig. S1b. (c) Paraffin-embedded cells (from a and b) were probed with the C219 antibody (top row, green staining) or RNAscope probes to detect zebrafish abcb4 (middle row, magenta staining) or zebrafish abcb5 (bottom row, yellow staining), as outlined in Materials and Methods. Bar = 100 µm.