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. 2021 Dec 17;13(1):5. doi: 10.1038/s41419-021-04442-9

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© The Author(s) 2021, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — C57BL/6 mice were treated with CCl₄ for 6 weeks and subsequently injected with GalN. All animals were sacrificed 48 h after GalN injection. A GalN injection was associated with a significant increase of ALT levels. B Caspase 3/7 and 8 expression showed a mixed response without proving significance. C TUNEL staining of liver tissue showed that GalN injection induced hepatocyte cell death with high expression of RIPK3 (magnification, 10x and 40x). RIPK1 inhibition by RIPA 56 significantly reduced ALT levels, abrogated tissue injury (TUNEL staining) and reduced the liver RIPK3, pMLK, cleaved-Caspase 3 and 8 expression. The optical density of immunostaining intensity (mean ± SEM) was measured. Data were analyzed using Student’s t test or Mann–Whitney U test. Only significant p values were displayed *p < 0.05, **p < 0.01, ***p < 0.001.