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. 2021 Dec 17;12:7336. doi: 10.1038/s41467-021-27607-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Experimental workflow of in vivo experiments. b Protein levels of indicated soluble signals in wildtype (n = 7) and Osm^−/⁻ (n = 7) tumours, measured by ELISA. Results displayed as mean ± SEM, two-tailed student t test. c Immunohistochemical analysis of αSMA in wildtype and Osm^−/⁻ tumour sections. Left: Representative αSMA staining. Right: Quantification of αSMA^pos staining of tumour sections of wildtype (n = 6) and Osm^−/− (n = 6) animals. Results displayed as mean ± SEM, two-tailed student t test. d RT-qPCR gene expression analysis of FACS-isolated CAFs from wildtype (n = 5) and Osm^−/− (n = 5) tumours displayed as a heatmap (left). Number of isolated CAFs shown as bar plot (right). Results displayed as mean ± SEM, two-tailed student t test. e Immunohistochemical analysis of CD8 in wildtype (n = 6) and Osm^−/− (n = 6) animals. Upper: Total CD8^pos staining. Lower: Ratio of peripheral/central CD8^pos staining using the mean CD8^pos stain from four representative peripheral regions and four representative central regions per tumour. Results displayed as mean ± SEM, two-tailed student t test. f Mass cytometry analysis quantifying abundance of antigen presenting cells (APCs) expressing co-stimulatory markers in wildtype (n = 5) and Osm^−/− (n = 5) tumours. Results displayed as mean ± SEM. Unpaired two-tail student t test. g Mass cytometry analysis of CD45⁺/CD3⁺ T cells expressing indicated markers in wildtype (n = 5) and Osm^−/− (n = 5) tumours. Results displayed as mean ± SEM, two-tailed student t test. h, i Mass cytometry analysis of memory T cells expressing indicated exhaustion/dysfunction markers in wildtype (n = 5) and Osm^−/− (n = 5) tumours. Results displayed as mean ± SEM, two-tailed student t test. Tcm central memory, Tem effector memory, Trm resident memory. j Spearman correlation analysis between infiltration of indicated immune cells (determined by CIBERSORT) and OSMR expression levels in PDA patients from TCGA PanCancer PAAD dataset (n = 179). 95% confidence interval as shaded area. Also see Supplementary Figs. S5 and S6. Source data are provided as a Source Data file.