Table 1.

Effect of elicitor treatments on apigenin contents.

Elicitors types	Elicitors concentrations	A. trigonus Calli		A. trigonus Cell suspension
		Mean apigenin content (mg/g)	Apigenin change (fold) compared with the control	Mean apigenin content (mg/g)	Apigenin change (fold) compared with the control
Control (MS free)	0	0.801 ± 0.04	1.00	0.951 ± 0.060	1.00
SA (µM)	50	0.769 ± 0.030	0.96	1.361 ± 0.300	1.43
	100	0.897 ± 0.060	1.12	1.658 ± 0.200	1.74
	150	0.467 ± 0.040	0.58	0.965 ± 0.040	1.01
	200	0.384 ± 0.009	0.48	0.364 ± 0.030	0.38
YE (mg/l)	50	1.951 ± 0.090	2.43	2.198 ± 0.100	2.31
	100	2.786 ± 0.400	3.48	3.793 ± 0.100	3.99
	150	1.724 ± 0.300	2.15	2.483 ± 0.300	2.61
	200	1.396 ± 0.300	1.74	1.978 ± 0.100	2.08
CH (mg/l)	50	1.860 ± 0.090	2.32	0.325 ± 0.070	0.34
	100	0.191 ± 0.020	0.24	2.048 ± 0.300	2.15
	150	0.101 ± 0.020	0.13	0.248 ± 0.080	0.26
	200	0.654 ± 0.120	0.82	0.785 ± 0.120	0.83
JA (µM)	50	0.185 ± 0.040	0.23	0.245 ± 0.020	0.26
	100	1.416 ± 0.150	1.77	2.047 ± 0.080	2.15
	150	0.921 ± 0.040	1.15	2.102 ± 0.120	2.21
	200	1.621 ± 0.100	2.02	1.782 ± 0.100	1.87

HPLC was used for measuring the apigenin concentration in the six weeks A. trigonus calli and cell suspension cultures incubated on MS media supplemented with different concentrations of SA, YE, JA and CH.