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. 2021 Dec 17;11:24191. doi: 10.1038/s41598-021-03638-5

Figure 3.

Figure 3

Immunohistochemical findings in naturally Usutu virus (USUV) infected birds. (A) USUV antigen was detected in the cytoplasm of a high numbers of cells, mostly macrophages, in both red and depleted white pulp in the spleen of a great grey owl (Supplementary Table S1, #1; scale bar: 50 µm; U433). (B) The Bursa fabricii of a blackbird (Supplementary Table S1, #2) showed low to moderate amounts of viral antigen, frequently located in the periphery of follicles (scale bar: 50 µm; U433). (C) The liver of a great grey owl (Supplementary Table S1, #1) presented moderate amounts of viral antigen multifocally in the cytoplasm of necrotic and intactly appearing hepatocytes as well as in Kupffer cells (scale bar: 50 µm; U433). (D) The cerebellum of a blackbird (Supplementary Table S1, #7) showed high amounts of viral antigen predominantly within Purkinje cells and glial cells (scale bar: 50 µm; U433). (E) In the cerebrum of a blackbird (Supplementary Table S1, #7) high numbers of unaltered neurons contained USUV antigen (scale bar: 50 µm; U433). (F) The lung of a great grey owl (Supplementary Table S1, #1) yielded low to moderate amounts of viral antigen in the interstitium and multifocally within lumina of small and large blood vessels (scale bar: 50 µm; U433).