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. 2021 Dec 17;11:24194. doi: 10.1038/s41598-021-03679-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Neutrophil depletion treatment after 15 weeks of high fat-fructose-cholesterol diet (FFC). (a) Mice were fed with FFC diet for 15 weeks and were administered with IgG (FFC + IgG, n = 6) or anti-Ly6G (FFC + anti-Ly6G, n = 6) intraperitoneal injections for 14 days until sacrifice. Control diet was used as a NASH negative control (CD, n = 6). Liver/body weight ratio (b), liver weight (c), body weight (d) and serum alanine transaminase (e) were compared between the mentioned groups with 1-way analysis of variance (ANOVA) followed by the Turkey post hoc test. P < .05 was considered a significant difference. Ly6G lymphocyte antigen 6 complex locus G6D, LW/BW liver/body weight ratio, ALT alanine aminotransferase.