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. 2021 Dec 17;11:24194. doi: 10.1038/s41598-021-03679-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Hepatic steatosis, fibrosis and hepatic stellate cell (HSC) activation are ameliorated in neutrophil depleted mice during NASH development. Neutrophil marker Ly6G (a), steatosis (b) and fibrosis (c) levels were assessed via IHC, H&E and Picrosirius Red stainings IHC respectively on paraffin-embedded tissue sections. Activated α-SMA⁺ HSCs (d) were detected via IHC. Quantification and ANOVA comparison were made between the control diet and IgG or anti-Ly6G regimen receiving groups during ongoing NASH development. (e) mRNA expression of hepatic fibrosis and HSC activation related genes and hydroxyproline levels were analyzed between the mentioned groups and compared with ANOVA IHC. CD control diet, α-SMA/acta2 alpha smooth muscle actin, IHC immunohistochemistry, col3a1 collagen type 3 alpha 1 chain, timp1 tissue inhibitor of metalloproteinases 1, tgfb transforming growth factor beta, ctgf connective tissue growth factor.