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. 2021 Dec 17;11:24194. doi: 10.1038/s41598-021-03679-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Hepatic steatosis, fibrosis and hepatic stellate cell (HSC) activation are ameliorated in NASH resolution induced by diet reversal. Steatosis (a), fibrosis (b) and activated HSC activation (c) were detected via hematoxylin, Sirius Red and α-SMA IHC stainings respectively in paraffin embedded tissue sections. Hydroxyproline levels and mRNA expression of fibrosis-related genes (d) and HSC activation markers (e) were analyzed through RT-qPCR among the control diet, FFC and diet reversal receiving groups. Data was analyzed with ANOVA comparison followed by the Turkey post hoc test.