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. 2021 Dec 17;13(1):4. doi: 10.1038/s41419-021-04174-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Correlation map of expression data of OLR1 and c-MYC in GSE10950, GSE41328, and GSE75970. B Immunohistochemical staining to detect the expression of c-MYC protein in colon cancer tissues and adjacent normal tissues (adjacent tissues: n = 120; tumor tissues: n = 120). C RT-qPCR and western blot analysis to detect the expression of OLR1 and c-MYC in colon cancer cells. D Detection of Ki67 mRNA expression in colon cancer cells by RT-qPCR. E MTS to detect the proliferation of colon cancer cells. F Colony formation assay to detect the colony formation of colon cancer cells. G The drug resistance of colon cancer cells to DDP, oxaliplatin, 5-Fu, and paclitaxel detected by MTS. H RT-qPCR to detect the mRNA expression of P-gp and SMAD4 in colon cancer cells. The cell experiment was repeated three times. *p < 0.05 between two groups.