| PCR cycling conditions | |||
|---|---|---|---|
| Steps | Temperature | Time | Cycles |
| Initial Denaturation | 95 °C | 3 min | 1 |
| Denaturation | 95°C | 30 s | 34 cycles |
| Annealing | 62 °C | 30 s | |
| Extension | 72 °C | 3 min | |
| Final extension | 72 °C | 3 min | 1 |
| Hold | 4 °C | Unlimited | |
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