Reagent | Final concentration | Amount |
---|---|---|
RPMI 1640 Medium | n/a | 500 mL |
Heat-Inactivated Fetal Bovine Serum (FBS) | 10% | 50 mL |
SC+ supplement | 5% | 25 mL |
Total | n/a | 575 mL |
Store at 4 °C for several weeks.
Reagent | Final concentration | Amount |
---|---|---|
RPMI 1640 Medium | n/a | 500 mL |
Heat-Inactivated Fetal Bovine Serum (FBS) | 10% | 50 mL |
SC+ supplement | 5% | 25 mL |
Total | n/a | 575 mL |
Store at 4 °C for several weeks.