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. 2021 Dec 14;3(1):101031. doi: 10.1016/j.xpro.2021.101031

Complete RPMI medium

Reagent Final concentration Amount
RPMI 1640 Medium n/a 500 mL
Heat-Inactivated Fetal Bovine Serum (FBS) 10% 50 mL
SC+ supplement 5% 25 mL
Total n/a 575 mL

Store at 4 °C for several weeks.