Sucrose buffer (pH 7.4)

Reagent	Final concentration	Amount
Tris-HCl (1 M)	25 mM	1.25 mL
MgCl2 (1 M)	5 mM	0.25 mL
NaCl (4 M)	100 mM	1.25 mL
DEPC H2O	n/a	up to 50 mL
Total	n/a	50 mL

This buffer can be stored at 4°C up to one year.

The 100 μg/mL cycloheximide, 40 U/mL RNase inhibitor and 1 mM PMSF should be added before use.