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. 2021 Nov 15;298(1):101403. doi: 10.1016/j.jbc.2021.101403

Figure 4.

Figure 4

Thermal unfolding of DARPin domains followed by CD spectroscopy between 40 °C and 95 °C; all variants were measured at a concentration of 10 μM in PBS.A, N1C_v01 to N1C_v04: The measured T m values were 74.5 °C for the control N1C (N1C_v01), 64.7 °C for the N-Cap Leu4Ala mutant (N1C_v02), 74.3 °C for the N-Cap Gly5Ala mutant (N1C_v03), and 82.4 °C for the N-Cap Asp17Ala mutant (N1C_v04). B, the Asp17Leu mutant (N1C_v05) has a T m of 84.6 °C and is strongly stabilized compared with N1C_v01 containing Asp17. DARPin, designed ankyrin repeat protein.