Table 1.

Description of DARPin domains used in the present study with varying N- and C-Caps, as detailed in the text

Domain name	Description
N1C	DARPin domain with one full consensus IR corresponding to NI1C of Interlandi et al. (24) & Wetzel et al. (26).
aHER2	DARPin domain based on an anti-HER2 DARPin domain corresponding to H10-2-G3 (“G3”; Zahnd et al. (20)).
aVEGF	DARPin domain based on an anti-VEGF-A DARPin domain of the protein moiety of abicipar pegol (28).
aHSA	DARPin domain based on the anti-HSA DARPin domain of ensovibep (10). It possesses an improved C-Cap similar to the mut5 C-Cap described by Interlandi et al. (24).

For a comprehensive list of amino acid sequences of all domain variants used, see Table S1.