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Tm values of N1C variants having either Asp or Leu at position 17 of the N-Cap in wt and mut5 C-Cap backgrounds
| Name | N-Cap | C-Cap | Tm (°C) | Tma (°C) |
|---|---|---|---|---|
| N1C_v16 | N01 | wt | 62.1 | ND |
| N1C_v25 | N01 | mut5 | 74.6 | ND |
| N1C_v01 | N02 | wt | 74.5 | ND |
| N1C_v22 | N02 | mut5 | 84.2 | 67.3 |
| N1C_v23 | N02_D17L | mut5 | ND | 79.3 |
Abbreviation: ND, not determined.
Indicates Tm measurements in 2 M GdmCl.
