Fig. 1.

Histological changes in the mouse knee and assessment of osteoarthritis using OARSI scores, as previously shown in Steinbusch et al., 2017 [13]. (a) Histological images showing the medial femoral condyle (above) and medial tibial plateau (below) for young, old, Sham and DMM mice knees. Safranin-O with Fast-Green counterstain; red indicates proteoglycan. Scale bar 100 ​μm. (b/c) Assessment of osteoarthritis using OARSI scores in young (n ​= ​8) vs. old (n ​= ​4) (b); and in Sham (n ​= ​5) vs. DMM (n ​= ​6) (c). Data represents the mean ​+ ​95% confidence interval (CI) for each scorer. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)