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. 2021 May 27;3(4):100186. doi: 10.1016/j.ocarto.2021.100186

Fig. 3.

Fig. 3

Validation of differentially expressed microRNAs between young vs. old samples following small RNA sequencing in discovery (a) and validation (b) cohorts using RT-qPCR. Histograms of relative expression calculated using 2^-DCT method using U6 as an endogenous control. Bars represent means with standard deviation. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001. (c) RT-qPCR results for two of the differentially expressed microRNAs between Sham vs. DMM samples following small RNA sequencing in the discovery cohort. Histograms of relative expression calculated using 2^-DCT method using U6 as an endogenous control. Bars represent means with standard deviation.