Preparing complex I in-gel activity solution

Composition of the complex I in-gel activity solution
Reagent	Final concentration	Amount
10 mg/mL Nitrotetrazolium Blue	2.5 mg/mL	1.25 mL
40 mM NADH	0.136 mM	0.017 mL
10 mM Tris-HCl, pH 7.4	5 mM	2.5 mL
Ultrapure Water		1.233 mL
Total		5 mL

Storage conditions: Make just before you begin, and keep on ice.