Preparing complex IV in-gel activity solution

Composition of the complex IV in-gel activity solution
Reagent	Final concentration	Amount
1% Diaminobenzidine	0.05%	0.25 mL
0.83 mM Cytochrome C	0.05 mM	0.3 mL
100 mM Sodium Phosphate, pH 7.2	50 mM	2.5 mL
Ultrapure Water		1.95 mL
Total		5 mL

Storage conditions: Make just before you begin, and keep on ice.