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Preparing 20× BN PAGE running buffer
| Composition of the 20× BN PAGE running buffer | ||
|---|---|---|
| Reagent | Final concentration | Amount |
| Bis-Tris | 1 M | 209.2 g |
| Tricine | 1 M | 179.2 g |
| ddH2O | Adjust volume to 1000 mL | |
| Total | 1000 mL | |
Store up to 6 months @ room temperature (25°C).
