Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Preparing 1% digitonin buffer
| Composition of the 1% digitonin buffer (D) | ||
|---|---|---|
| Reagent | Final concentration | Amount |
| 4× Sample Buffer | 1× | 62.5 μL |
| 5% Digitonin | 1% | 50 μL |
| 100× Protease Inhibitor | 1× | 2.5 μL |
| Ultrapure Water | 135 μL | |
| Total | 250 μL | |
Storage conditions: Make just before you begin, and keep on ice.
