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Preparing G-250 blue dye
| Composition of the blue dye (B) | ||
|---|---|---|
| Reagent | Final concentration | Amount |
| 4× Sample Buffer | 2.67× | 100 μL |
| 5% G-250 Sample Additive | 0.42% | 12.5 μL |
| Ultrapure Water | 37.5 μL | |
| Total | 150 μL | |
Storage conditions: Make just before you begin, and keep on ice.
