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. 2021 Dec 18;21:351. doi: 10.1186/s12866-021-02401-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — A western blot for the purified rSARS-CoV-2-N protein. The prestained protein marker and purified recombinant proteins were separated by SDS-PAGE and transferred to a PVDF membrane. Each membrane was incubated with diluted patient’s serum or mouse immune serum, followed by horseradish peroxidase conjugated-goat anti-human IgG or anti-mouse IgG (1:1000 dilution), and detected by DAB staining. (A) Reactivity of recombinant proteins to COVID-19 patient serum (1:400 dilution). Lane 1: protein marker; Lane 2: purified rSARS-CoV-2-N protein. (B) Reactivity of recombinant proteins to rSARS-CoV-2-N-immunized mouse serum (1:800 dilution). Lane 1: protein marker; Lane 2: purified rSARS-CoV-2-N protein