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. 2021 Dec 18;54:40. doi: 10.1186/s40659-021-00363-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — IL-6 treatment counteracted and wp1066 imitated the anti-proliferative activity of diosmetin in osteosarcoma Saos-2 and U2SO cells. Saos-2 or U2SO cells were treated with diosmetin with or without IL-6 for 24 h, replaced with fresh culture medium and cultured for 2 weeks, stained with crystal violet and photographed. A Representative images of colonies of Saos-2 and U2SO cells from six-well plates in which cells were treated with DMSO control, diosmetin or diosmetin + IL-6 using colony formation assay. B Quantification of colony number in DMSO-, diosmetin- or diosmetin + IL-6-treated Saos-2 and U2SO cells. C Representative images of colonies of Saos-2 and U2SO cells from six-well plates in which cells were treated with DMSO control, WP1066, diosmetin or WP1066 + diosmetin using colony formation assay. D Quantification of colony number in DMSO-, WP1066, diosmetin- or WP1066 + diosmetin—treated Saos-2 and U2SO cells. The results are shown as the mean ± SD of three independent experiments (n = 3, *P < 0.05, ***P < 0.001)