Fig. 5.

Triptonide-mediated degradation of Twist1 in TNBC cells was not dependent on the ubiquitin–proteasome system. After MDA-MB-231 and MDA-MB-468 cells were treated with or without 10 nM triptonide (TN) and with or without 20 μM of the ubiquitin–proteasome inhibitor MG132, Twist1 protein levels were detected by Western blotting (A, C) and statistically analyzed (B, D). The results showed that TN-mediated degradation of Twist1 protein in these cells was not significantly changed, suggesting that TN-mediated degradation of Twist1 is not dependent on the ubiquitin–proteasome system. The results represent three independent experiments. *P < 0.05, **P < 0.01