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. 2021 Dec 18;19:425. doi: 10.1186/s12951-021-01167-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — A Zeta potential of GCL/DOPE/DSPE-PEG/DNA at different Lipid/DNA mass ratios (mL/mD). (mL/mD)_Φ, is the electroneutrality value. Black open circles for ctDNA and red circles for MSCV-OPA1. B SAXS diffractograms of GCL/DOPE/DSPE-PEG mixed lipids. Inset: q-values vs. ${(h^{2} + k^{2} + l^{2})}^{1 / 2}$ for the Ia3d phase. C SAXS diffractograms of GCL/DOPE/DSPE-PEG/OPA1 at ρ_eff = 2.5. Inset: q-values vs. ${(h^{2} + k^{2} + l^{2})}^{1 / 2}$ for two different Ia3d phases (red and white symbols). D SAXS diffractograms of GCL/DOPE/DSPE-PEG/OPA1 at ρ_eff = 4. Inset: q-values vs. ${(h^{2} + k^{2} + l^{2})}^{1 / 2}$ for two different Ia3d phases (red and white symbols)