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. 2021 Dec 18;19:425. doi: 10.1186/s12951-021-01167-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Western blot analysis of Opa1 and GRP75 protein levels in MEFs wt (A) and OPA1-KO MEFs (B) after transfection with (C₁₆Im)₂(C₄O)/DOPE/DSPE-PEG/OPA1 lipoplexes at ρ_eff = 2.5 and 4 at different time intervals. The relative ratios of band intensity between Opa1 and GRP75 (lower panels) were quantified by ImageJ. Student’s t-test was performed to measure the significance of statistical difference between the different groups and the control (in the absence of treatment) from 3 independent experiments. (*p < 0.05 was considered statistically significant)