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. 2021 Dec 18;19:425. doi: 10.1186/s12951-021-01167-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — A Western blot of Opa1 and GRP75 protein levels in kidneys and brain from CD-1 mice after 48 h of transfection with (C₁₆Im)₂(C₄O)/DOPE/DSPE-PEG/OPA1 lipoplexes (ρ_eff = 4). Different lanes correspond to the groups without treatment (C) and with treatment via intraperitoneal (IP), intracardiac (IC) and intramuscular (IM) administration. B Relative ratios of band intensity between Opa1 and GRP75 from different organs of CD-1 mice treated with (C₁₆Im)₂(C₄O)/DOPE/DSPE-PEG/OPA1 lipoplexes (ρ_eff = 4) via intraperitoneal (IP), intracardiac (IC) and intramuscular (IM) administration. Results shown are mean ± s.e.m. from 3 independent experiments. The statistical significance was determined using a Student’s t-test between the different groups and the negative control (in the absence of treatment); (*p < 0.05 was considered statistically significant)