Fig. 7.

TRIM29 is a downstream target of miR-761. a Overlapping of the target genes of miR-761 predicted by cross-analysis of starbase3.0, RNA22, miRWalk and miRTarBase. b Heatmap of top 25 overexpressed genes in the TCGA-COAD database. c TRIM29 expression at the mRNA level in CRC tumors and corresponding normal tissues from the TCGA database. d TRIM29 expression in the primary tumors and normal tissues of CRC patients from the GEO database. e and f The mRNA and protein levels of TRIM29 were assessed by qRT-PCR and western blot in CRC cells transfected with miR-761 mimics or inhibitor. g Schematic illustration of WT and Mut TRIM29 luciferase reporter vectors. h and i CRC cells were cotransfected with miR-761 mimics and either WT or Mut TRIM29 luciferase reporter vectors, and the activity of the luciferase reporter was determined. j and k qRT-PCR analysis showed that TRIM29 expression was significantly upregulated in 120 CRC tissues compared with the paired ANTs. l and m TRIM29 expression in CRC was positively correlated with circIL4R and negatively correlated with miR-761 based on qRT-PCR. *P < 0.05, **P < 0.01, ***P < 0.001