Xanthatin inhibited retinoblastoma growth in vivo. (a) TG(flk:eGFP) embryos (2 dpf) injected with Y79 cells with red fluorescence for easy observation. Zebrafish was treated with xanthatin (0 and 5 µM), n = 10. Six days after treatment, image capture, processing, and adjustment were performed. Scale bars, 200 µm for magnification images. (b) Statistical analysis showed that the fluorescence of the 5 µM xanthatin group was lower than that of the 0 µM xanthatin group. The P values are from unpaired t test, ****P < 0.0001.