Figure 4. Maximum likelihood phylogenetic trees and protein alignments from two VLCs with domains shown as colored blocks.

(A) Tree of C-type lectin-like protein has topology in agreement with established cnidarian taxonomy. (B) Tree of astacin-like transcript has topology incongruent with established cnidarian taxonomy and includes sequences from venomous animals outside of Cnidaria. See Additional File 3 for trees of all candidate VLCs.