Table 1.
Characteristics of replicons identified in OCU_Ac16a
Replicon | Size (bp) | Antimicrobial resistance genes | rep gene group of plasmids | Elements related to plasmid mobility | Frequencies of plasmid transfera |
---|---|---|---|---|---|
Chromosome | 3 992 063 | bla TMB-1, blaADC-25-like, blaOXA-51-like, strA, strB, mph(E), msr(E), sul1, sul2, gyrA (S81L) | – | – | – |
pOCU_Ac16a_1 | 73 028 | ND | GR6 | MOBF family relaxase, TraD-like T4CP, MPFF | – |
pOCU_Ac16a_2 | 41 087 | bla NDM-1, aph(3′)-VIa | ND | oriT, MOBQ family relaxase, TrwB-like T4CP, MPFT | 2.5 × 10−5–1.5 × 10−2 (A. baumannii ATCC 19606T RFP50R)b; 2.2 × 10−7–5.4 × 10−1 (Acinetobacter strains OCU_Ac4, 8, 9, and 12 RFP50R) |
pOCU_Ac16a_3 | 13 096 | bla OXA-58 | GR4 | ND | ND (A. baumannii ATCC 19606T RFP50R) |
ND, not detected; –, not applicable or not tested; GR, homology group; MOB, mobilization; T4CP, type IV coupling protein; MPF, mating pair formation; oriT, origin of transfer.
The recipient strains used are indicated in parentheses.
Results from four independent experiments.