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. 2021 Dec 11;2021:6521953. doi: 10.1155/2021/6521953

Figure 4.

Figure 4

Transfection efficiency of TBK1si/rGO-PEG and the expression of TBK1. TBK1si/rGO-PEG was used to transfect U251 cells for 3 hours. The transfection efficiencies at different weight ratios. (A) NS control and rGO-PEG vs. TBK1si as 1 : 5 (B), 1 : 50 (C), and 1 : 500 (D) accessed by the fluorescence microscope (a) and flow cytometry (b). When U251 cells were transfected with NS, rGO-PEG, Consi/rGO-PEG, or TBK1si/rGO-PEG for 3 days, TBK1 was investigated by RT-PCR (c) and Western blot (d). NS, normal saline. The result was a representative of three independent experiments. P values were determined by one-way analysis of variance (ANOVA) followed by Tukey post hoc test. ∗∗∗P < 0.001.